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. 2009 Mar 20;284(12):8174–8184. doi: 10.1074/jbc.M805787200

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FIGURE 5. — Polyamine synthesis and uptake after siRNA knockdown of AZI. A, real-time RT-PCR analysis of AZI mRNA levels after siRNA knockdown. Alveolar macrophages from Dex and Dex-Pc rats were transfected with AZI siRNA (siAZI) or scrambled siRNA (Scrambled) and then measured for AZI mRNA levels. B, the ODC protein level in alveolar macrophages from Dex-Pc rats was determined by Western blotting after transfection with AZI siRNA (siAZI) or scrambled siRNA (Scr). C, alveolar macrophages from Dex-Pc rats were assayed for FAM-SPD uptake after transfection with AZI siRNA (siAZI) or scrambled siRNA (Scr). A total of 1000 cells of each sample was counted, and the percentages of cells with internalized FAM-SPD were determined and diagrammed.