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. 2009 Mar 1;23(5):619–632. doi: 10.1101/gad.1760209

Figure 4.

Figure 4.

Loss-of-muscle miRNAs affects the gene expression pattern of targets mRNAs. (A,B) Relative expression of miR-1 target and nontarget mRNAs in muscle versus nonmuscle tissue. Plotted is the fraction of genes (Y-axis) with a relative expression in muscle versus nonmuscle (X-axis) in wild-type (A), and miR-1 MO knockdown (B). Genes with muscle miRNA target sites up-regulated in both MZdicer+miR-430 and miR-1 MO experiments (orange). Genes that lack a 7mer target sites for miR-1 or miR-133 in their 3′UTR (black). Note that the expression bias of muscle miRNA targets (orange) toward low in muscle is strongly reduced when miR-1 is knocked down. (C) qRT–PCR of target mRNAs and a control mRNA (myoD) in muscle cells after FACS sorting of wild-type or miR-1 MO knockdown embryos. (D–F) In situ hybridization (purple) for miR-1 target genes in control MO-injected embryos (wild type) or miR-1 knockdown embryos (miR-1 MO). The insets show the enlarged view of the dissected muscle. Note the moderate increase in the expression levels of the targets when miR-1 is inhibited (arrowhead and insets).

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