Figure 6.

DDK–Dbf4^Δ110-296 is an active kinase but cannot bind the Mcm2–7 complex or phosphorylate Mcm4 or Mcm6. (A) Dbf4^Δ110–296 mutant. The Dbf4 mutant used in this figure is depicted. Regions required for Mcm2–7 and Cdc7 interaction are indicated (Varrin et al. 2005). (B) DDK–Dbf4^Δ110–296 has autophosphorylation activity but does not phosphorylate Mcm4 and Mcm6. The experiment was performed as described in Figure 2A. (*) Detection of nonspecific proteins in lanes 1 and 3 of Western; (●) origin-independent phosphoprotein. (C) DDK–Dbf4^Δ110–296 is defective for pre-RC association. DDK and DDK–Dbf4^Δ110–296 association with pre-RCs was measured as described in Figure 5A.