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. 2009 Feb 6;10(3):278–284. doi: 10.1038/embor.2009.4

Figure 4.

Figure 4

PEBP4 regulates myoblast differentiation. (A) SkMC myoblasts were co-transfected with DsRed mito plasmid and pcDNA3-PEBP4, pSUPER-PEBP4 shRNA or the respective empty vectors as indicated. FACS-sorted cells were placed in a differentiation medium (DM) and examined 3 days later for myosin heavy chain (MyHC) expression (green) and myotube formation as markers for terminal differentiation. Nuclei were stained with DAPI (blue). Results shown are representative of three independent experiments with ⩾4,000 nuclei counted per experiment. (B) SkMC myoblasts were co-transfected with vector or pcDNA3-PEBP4 and induced to differentiate. Lysates were blotted for the differentiation markers, myogenin and p21CIP/WAF. Blots were quantified by laser densitometry (lower panel). Results are representative of three independent experiments. DAPI, 4,6-diamidino-2-phenylindole; FACS, fluorescence-activated cell sorting; PEBP4, human phosphatidylethanolamine-binding protein 4; shRNA, short hairpin RNA.