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. 2009 Feb 13;10(3):258–263. doi: 10.1038/embor.2009.6

Figure 2.

Figure 2

NS3 has no interferon and PKR antagonistic properties. HEK293T cells were co-transfected with expression vectors encoding firefly luciferase under the control of an IFN-β-inducible promoter, Renilla luciferase, and VP35, NS3 (10, 100 and 400 ng) or pBluescript (C), either in the presence (+) or absence (−) of poly I:C. Luciferase expression was measured 3 days post-transfection. A relative luciferase expression corrected for the internal Renilla control (firefly/Renilla) is shown. The mean of at least three independent experiments is shown with standard error. HEK, human embryonic kidney; IFN, interferon; NS3, non-structural protein 3; PKR, protein kinase R.