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. 2009 Jan 30;10(3):264–270. doi: 10.1038/embor.2008.247

Figure 4.

Figure 4

Dual targeting by miR390, but not miR159, can trigger the formation of small interfering RNA. (A) Construct diagrams (left), fraction of transgenic plants with bleaching phenotype (middle) and 5′ RACE results (right). CH42 sequences are indicated in red and miRNA target sites in green or blue. Cleavage at the downstream miR390 target site could only be mapped using a modified RACE procedure (see Methods). (B) Plants expressing the various CH42 silencing reporters. (C) Small RNA analysis of plants expressing CH42 reporters. CH42, CHLORINA 42; RACE, rapid amplification of cloned ends.