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Effect of BEPP on PKR, eIF2α, AKT, cyclin D1, and MAPK activity. MEF/PKR(+/+) and MEF/PKR(-/-) cells were treated with the indicated concentrations of BEPP for 72 h. Whole-cell lysates were analyzed for total and active (phosphorylated) PKR, eIF2α, and cyclin D1, total and active (phosphorylated) AKT, active p38, and JNK by Western blotting. β-Actin was used as a loading control.
