Fig. 6.

Effect of BEPP on vSP replication in HeLa cells. A, cells were pretreated with 7.5 μM BEPP for 48 h. DMSO pretreatment was used as a control, and BECC pretreatment was used as a compound control. Cells were then infected with vSP at a dose of 0.2 MOI. Cell lysates were harvested at the indicated time points and titrated using the TCID₅₀ method. The virus yield in the BEPP group was much lower than in the DMSO or BECC group (p < 0.01) at days 4 to 6. B, cell lysates were harvested after infection of vSP for 4 days. The lysates were analyzed for total and active (phosphorylated) PKR and eIF2α by Western blotting.