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. Author manuscript; available in PMC: 2010 Feb 15.
Published in final edited form as: J Immunol. 2009 Feb 15;182(4):1868–1876. doi: 10.4049/jimmunol.0802501

Figure 1. Preferential loss of CD4 memory in B cell-deficient mice.

Figure 1

B cell-deficient (µMT−/−) and +/+ mice were infected with 2 × 105 PFU of LCMV (strain Armstrong), and LCMV-specific CD8 and CD4 T cell responses were quantified in the same mice by intracellular IFN-γ staining. Primary responses were measured at day 8 after infection, and memory responses were measured at days 70–154. (A) An example of the CD8 response to LCMV peptide GP33-41 with numbers indicating the frequency of CD8+ T cells that are GP33-specific. (B) The number of GP33-specific CD8+ T cells per spleen at various times after infection. (C) The CD4 response at days 8 and 154 with numbers indicating the frequency of CD4 T cells specific to GP61-80. (D) The number of GP61-80 specific CD4 T cells per spleen at day 8 or memory time points. In panels B and D, the numbers in parenthesis indicate the fold reduction in cell number between primary and memory time points. The dashed lines depict the limits of detection by this assay, and error bars represent standard deviations of 3 mice from a representative experiment.