Representative cholera toxin beta fragment (CTβ) and gold conjugated cholera toxin beta fragment (CTβG) injection sites. Areal borders were determined from adjacent Nissl and SMI-32 antibody-stained sections (Lee and Winer, 2008a). A–C: CTβ injections spread 500–1000 µm. Two injections were usually made to ensure adequate coverage in each area. Representative AI (A), AII (B), and DZ (C) deposits. D–F: CTβG deposits likewise were focal, and spread less than CTβ injections. To equalize coverage, an additional CTβG injection was made. Experiments with WAHG produced similar results. Deposits shown are in areas ED (D), EV (E), and AES (F). G–I: Both tracers were visualized in each section, as shown in areas Te (G), In (H), and P/VP (I). For orthogonal penetrations in areas DZ (C), AES (F), and P/VP (I), the sulcal banks were retracted.