Table 2.
Metabolites identified in the cell extracts of BY4742 and the used deletion mutants using the software AMDIS v2.0 and a TMS library.
| metabolite | peak number | m/z | #TMS/MeOx |
| glycine | 1 | 102 | 2 |
| alanine | 2 | 116 | 2 |
| pyruvic acid | 3 | 174 | 1/MeOx |
| lactic acid | 4 | 117 | 2 |
| valine | 5 | 144 | 2 |
| u. m. #1 | 6 | 110 | n.k.1 |
| ethanolamine | 7 | 174 | 2 |
| glycerol | 8 | 218 | 3 |
| isoleucine/leucine | 9 | 158 | 2 |
| succinic acid | 10 | 247 | 2 |
| uracil | 11 | 241 | 2 |
| fumaric acid | 12 | 245 | 2 |
| serine | 13 | 204 | 3 |
| threonine | 14 | 218 | 3 |
| homoserine | 15 | 218 | 3 |
| malic aicd | 16 | 233 | 3 |
| u. m. #2 | 17 | 188 | n.k.1 |
| erythritol | 18 | 217 | 4 |
| aspartic acid | 19 | 218 | 3 |
| u. m. #6 | 20 | 258 | n.k.1 |
| cytosine | 21 | 254 | 2 |
| α-ketoglutaric acid | 22 | 198 | 2/MeOx |
| glutamic acid | 23 | 246 | 3 |
| phenylalanine | 24 | 192 | 2 |
| ribose | 25 | 217 | 4/MeOx |
| asparagine | 26 | 231 | 3 |
| 2-aminoadipic acid | 27 | 260 | 3 |
| orotic acid | 28 | 254 | 3 |
| glycerol-1-phosphate | 29 | 370 | 4 |
| glutamine | 30 | 156 | 3 |
| N-acetyl-glutamic acid | 31 | 216 | 2 |
| u. m. #3 | 32 | 257 | n.k.1 |
| citric acid | 33 | 273 | 4 |
| ornithine | 34 | 258 | 4 |
| arginine | 35 | 256 | 5 |
| adenine | 36 | 279 | 2 |
| lysine | 37 | 230 | 4 |
| histidine | 38 | 356 | 3 |
| manitol | 39 | 307 | 6 |
| tyrosine | 40 | 218 | 3 |
| u. m. #4 | 41 | 258 | n.k.1 |
| inositol | 42 | 305 | 6 |
| xylulose-5-phosphate | 43 | 315 | 5/MeOx |
| glucose-6-phosphate | 44 | 387 | 6/MeOx |
| u. m. #7 | 45 | 387 | n.k.1 |
| u. m. #5 | 46 | 204 | n.k.1 |
| trehalose | 47 | 361 | 8 |
Peak numbers correspond to the labelling of the data points in Figure 2. #TMS denotes tert-methyl-silyl residues in the detected analyte originating from derivatization, MeOx denotes methoxymation of the metabolite. 1n.k., not known; u.m., unknown metabolite.