Table 2.
In silico comparison and comparative hybridization results for the two wbp gene clusters of PAO1
gene information | in silico analysis | comparative hybridization | ||||||||
count of similarity classesa | ||||||||||
PAO1 locus ID | PA14 locus ID | gene name | ident. | SNP | mism. | absent | total | average sequence similarity | detected oligosb | sensitivityb |
PA3141 | PA14_23470 | wbpM | 54 | 7 | 4 | 5 | 70 | 0.97 | 12 | 0.75 |
PA3142* | absent | 4 | 3 | 4 | 1 | 12 | 0.93 | 7 | 0.88 | |
PA3143* | absent | 4 | 4 | 8 | 3 | 19 | 0.90 | 11 | 0.73 | |
PA3144* | absent | 1 | 0 | 4 | 0 | 5 | 0.91 | 4 | 1.00 | |
PA3145* | PA14_23460 | wbpL | 0 | 0 | 3 | 33 | 36 | 0.71 | 35 | 0.97 |
PA3146* | PA14_23450 | wbpK | 0 | 0 | 6 | 28 | 34 | 0.71 | 33 | 0.97 |
PA3147* | absent | wbpJ | 0 | 0 | 6 | 37 | 43 | 0.72 | 43 | 1.00 |
PA3148* | absent | wbpI | 0 | 0 | 7 | 30 | 37 | 0.74 | 37 | 1.00 |
PA3149* | absent | wbpH | 0 | 0 | 6 | 33 | 39 | 0.72 | 39 | 1.00 |
PA3150* | absent | wbpG | 0 | 0 | 3 | 36 | 39 | 0.70 | 39 | 1.00 |
PA3151* | absent | hisF2 | 0 | 0 | 3 | 24 | 27 | 0.69 | 26 | 0.96 |
PA3152* | absent | hisH2 | 0 | 0 | 1 | 21 | 22 | 0.70 | 22 | 1.00 |
PA3153* | absent | wzx | 0 | 0 | 1 | 43 | 44 | 0.71 | 43 | 0.98 |
PA3154* | absent | wzy | 0 | 0 | 1 | 45 | 46 | 0.70 | 46 | 1.00 |
PA3155* | absent | wbpE | 0 | 0 | 5 | 33 | 38 | 0.72 | 38 | 1.00 |
PA3156* | absent | wbpD | 0 | 0 | 4 | 17 | 21 | 0.72 | 20 | 0.95 |
PA3157* | absent | 0 | 0 | 14 | 51 | 65 | 0.73 | 64 | 0.98 | |
PA3158* | absent | wbpB | 0 | 0 | 7 | 26 | 33 | 0.74 | 33 | 1.00 |
PA3159* | absent | wbpA | 0 | 0 | 14 | 32 | 46 | 0.73 | 46 | 1.00 |
PA3160 | PA14_23360 | wzz | 0 | 0 | 5 | 31 | 36 | 0.71 | 36 | 1.00 |
PA3161 | PA14_23340 | himD | 8 | 1 | 1 | 0 | 10 | 0.99 | 1 | 0.50 |
PA5447 | PA14_71910 | wbpZ | 35 | 4 | 1 | 0 | 40 | 0.99 | 5 | 1.00 |
PA5448 | PA14_71920 | wbpY | 34 | 5 | 0 | 0 | 39 | 0.99 | 2 | 0.40 |
PA5449 | PA14_71930 | wbpX | 40 | 8 | 1 | 0 | 49 | 0.99 | 4 | 0.44 |
PA5450 | PA14_71940 | wzt | 40 | 5 | 0 | 0 | 45 | 1.00 | 3 | 0.60 |
PA5451 | PA14_71960 | wzm | 24 | 5 | 0 | 0 | 29 | 0.99 | 3 | 0.60 |
PA5452 | PA14_71970 | wbpW | 42 | 6 | 1 | 0 | 49 | 0.99 | 4 | 0.57 |
* genes belonging to the PAO1 specific region.
a see Figure 2 for a definition of the similarity classes.
bdetermined for Dth = -0.5.