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Signal ratios of SNPs are biased towards the interaction type (strong or weak) of the bases present in the probe and sample alleles.
| PAO1 allele (probe)a | PA14 allele (sample)a | abundance | median signal ratio | sensitivityb |
| W | S | 15364 | -0.53 | 52.1% |
| W | W | 657 | -0.46 | 47.5% |
| S | S | 2592 | -0.38 | 43.7% |
| S | W | 12972 | -0.30 | 39.1% |
| Σ | 31585 | -0.43 | 45.9% | |
a W – IUPAC format for bases with weak interaction (A, T), S – strong interaction (G, C).
b determined for Dth = -0.5.
