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. Author manuscript; available in PMC: 2010 Feb 5.
Published in final edited form as: Virology. 2008 Nov 28;384(1):125–134. doi: 10.1016/j.virol.2008.10.011

Figure 3. Expression of HPV16 E5 increases the percentage of bi-nucleate cells.

Figure 3

A) tTA-HaCaT cells were infected with nothing or HA-E5 in the absence or presence of tetracycline (tet) as indicated. Cells were fixed and stained by indirect immunofluorescence with the 12CA5 mouse monoclonal antibody and nuclei were stained with DAPI. Arrow indicates a bi-nucleated cell. Images were collected using Olympus AX70 epifluorescent microscope with Q-Capture software. Size bar is 10 μm. B) The percentage of bi-nucleate cells treated as in (A). Shown is the average percentage ± S.E.M. of bi-nucleate cells from three independent experiments (≥500 cells/experiment). * indicates a p-value of <0.05 (two-tailed, unpaired t-test) as compared to time-matched cells infected in the presence of tetracycline.