Table 3.
Development of KO clones with reduced oocyst numbers in LacZ KD and CTL4 KD mosquitoes.
| dsLacZ (mature oocysts) | dsCTL4 (melanized parasites) | P-valuea | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Exp. | Mean | SEM | n | Prev. (%) | Mean | SEM | n | Prev. (%) | KO versus WT in dsCTL4 | dsLacZ versus dsCTL4 | |
| I | wt | 6 | 4 | 10 | 70 | 63 | 25 | 11 | 82 | – | n.s. |
| Δpsop2 | 0.35 | 0.13 | 20 | 30 | 2.06 | 0.73 | 18 | 39 | P < 0.01 | n.s. | |
| Δpsop7 | 0.04 | 0.04 | 24 | 4 | 0.00 | 0.00 | 7 | 0 | P < 0.01 | n.s. | |
| Δpsop9 | 16 | 4 | 40 | 78 | 26 | 7 | 34 | 94 | n.s. | n.s. | |
| II | wt | 11 | 6 | 11 | 45 | 18 | 5 | 18 | 83 | – | n.s. |
| Δpsop2 | 0.03 | 0.03 | 33 | 3 | 0.58 | 0.29 | 26 | 23 | P < 0.001 | n.s. | |
| Δpsop7 | 0.00 | 0.00 | 21 | 0 | 0.21 | 0.07 | 33 | 21 | P < 0.001 | n.s. | |
| Δpsop9 | 4 | 1 | 27 | 59 | 1 | 0 | 28 | 32 | P < 0.001 | P< 0.01 | |
a
Determined by Mann–Whitney U-test (n < 25) or z-test (n > 25). Exp., experiment; Prev., prevalence; n.s., not significant.