Fig. 1.

Targeting of CRE recombinase to the hypothalamus of transgenic mice. (a) The construct used to target CRE recombinase is illustrated. A −3446 bp fragment of the mouse gonadotrophin-releasing hormone (GnRH) promoter was fused upstream of CRE recombinase. CRE recombinase gene contains a Kozak initiation site, a nuclear localisation signal, an intron, derived from the SV40 t-antigen gene and a poly(A) signal. (b) Western blot analysis using an antibody specific for CRE protein in the indicated tissues from a GnRH-CRE mouse, founder line 35. Tissues shown are hypothalamus (hyp), cerebral cortex (ctx), cerebellum (cbl), liver (lvr), heart (hrt) and lung (lng).