Fig. 2.

CRE recombinase is expressed in gonadotrophin-releasing hormone (GnRH) neurones. Coronal section of a brain from a single 4-month-old male GnRH-CRE mouse (founder 35). Both sections are hypothalamic immediately caudal to the level of the organum vasculosum of the lamina terminalis. On the left is staining for Cre alone with DAB/Ni showing nuclear blue staining. On the right is an adjacent section stained for Cre (blue nuclear) and for GnRH (brown cytoplasmic). Both of these images are at × 100 magnification. Several neurones are boxed and shown in the inset at × 250 magnification. Scale bars = 100 μm. Cell counts from all brain sections from this mouse are also shown.