Fig. 3.

Gonadotrophin-releasing hormone (GnRH) neurones exhibit CRE recombinase activity. Coronal sections (× 100 magnification) from a bitransgenic GnRH-CRE/ROSALacZ mouse. Section includes the medial and lateral septum and the diagonal band of Broca (DBB). Tissue was treated with X-gal to visualise β-galactosidase activity, seen as blue. GnRH neurones were stained as above and are brown. A population of X-gal stained neurones are seen in the lateral septum and do not express GnRH. A group of GnRH expressing neurones are seen in the medial septum and DBB, all of which colocalise with X-gal staining. The boxed region is further shown at × 250 magnification.