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Amount of Each Solution to Add During Steps (c), (o), (q), and (s) When Using the Stated Coverslip Size
| Silane | Acrylamide solution | rBM solution | Ethanolamine | |
|---|---|---|---|---|
| 18-mm circle | 20 | 20 | 300 | 300 |
| 25-mm circle | 30 | 30 | 450 | 450 |
| 50-mm circle | 100 | 190 | 900 | 900 |
All values listed are in microliters.
