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Recipes of 1-ml Polyacrylamide Gel Solutions for Given Elastic Modulus (Yeung et al., 2005)
| Elastic modulus (Pa) | 140 | 400 | 1050 | 5000 | 60,000 |
| Acrylamide (%) | 3 | 3 | 3 | 5.5 | 10 |
| Bis-acrylamide (%) | 0.04 | 0.05 | 0.1 | 0.15 | 0.5 |
| 40% Acrylamide (μl) | 75 | 75 | 75 | 137.5 | 250 |
| 2% Bis-acrylamide (μl) | 20 | 25 | 50 | 75 | 250 |
| 0.5-M HEPES, pH 4.22 (μl) | 100 | 100 | 100 | 100 | 100 |
| TEMED (μl) | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
| ddH2O (μl) | 648.9 | 643.9 | 618.9 | 541.4 | 243.9 |
