Skip to main content
. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Nat Chem Biol. 2009 Jan 26;5(4):236–243. doi: 10.1038/nchembio.147

Figure 1.

Figure 1

Screening for chemical suppressors of AE. Homozygous Tg(hsp:AML1-ETO) fish were crossed with wild-type fish to generate thousands of heterozygous Tg(hsp:AML1-ETO) embryos. These embryos were raised for 12−16 hours post fertilization at which point five embryos were arrayed into each well of the 96-well plates. The compounds from the library were added to the plates. An hour later, the plates were heat-shocked at 40 °C for 1 hour to induce AE expression. At 90 minutes after the heat shock, the embryos were processed for in situ hybridization of gata1. Induced expression of AE resulted in lost of gata1+ hematopoietic cells (indicated as purple dots) in the posterior blood islands of the zebrafish embryos. However, the chemical suppressors of AE, such as nimesulide (shown in the figure), antagonized AE's effect, restoring gata1 expression in Tg(hsp:AML1-ETO) embryos.