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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Nat Chem Biol. 2009 Jan 26;5(4):236–243. doi: 10.1038/nchembio.147

Figure 2.

Figure 2

Nimesulide does not affect AE expression in Tg(hsp:AML1-ETO) embryos. Western blot analysis shows that while rotenone (0.15 μM) inhibits heat-induced expression of AE and Hsp70, neither nimesulide (40 μM) nor trichostatin A (TSA, 0.5 μM) affects heat-induced expression of AE and Hsp70. The expression of Akt is used as a reference for protein loading. At the concentrations used, all three compounds suppress the zebrafish AE phenotype. DMSO is used as the vehicle control. WT+, wild-type embryos with heat treatment. AE-, Tg(hsp:AML1-ETO) embryos without heat treatment. AE+, Tg(hsp:AML1-ETO) embryos with heat treatment. The vertical black line indicates the juxtaposition of lanes that were not contiguous in the original gel.