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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Nat Chem Biol. 2009 Jan 26;5(4):236–243. doi: 10.1038/nchembio.147

Figure 4.

Figure 4

The hematopoietic phenotype of AE involves induction of the genes that encode both zebrafish COX-2 isoforms. (a) Real-time PCR indicates that ptgs2b is upregulated in the hematopoietic cells of Tg(hsp:AML1-ETO) embryos at 2 hours after heat shock. Total RNA was extracted from the hematopoietic cells isolated from heat-treated wild-type and Tg(hsp:AML1-ETO) embryos at 22 hpf. The expression of ptgs1, ptgs2a, and ptgs2b was evaluated by real-time PCR analysis using two independent primer sets for each gene, and was normalized to gapdh expression. The fold change indicates the ratio of mRNA expression between Tg(hsp:AML1-ETO) and wild-type embryos. ptgs1, 2.09±0.0.91, 1.22±0.08; ptgs2a, 0.62±0.21; 1.04±0.03; ptgs2b, 10.31±1.39, 3.60±1.25 (mean±SEM). (b) Real-time PCR indicates that ptgs2a is upregulated in the hematopoietic cells of Tg(hsp:AML1-ETO) embryos at 40 hpf. ptgs1, 0.26±0.12, 0.28±0.11; ptgs2a, 3.86±0.36; 7.6±0.98; ptgs2b, 1.28±0.39, 1.45±0.81 (mean±SEM). (c) In situ hybridization of gata1. Knockdown of ptgs1, ptgs2a, or ptgs2b partially restored gata1 expression in heat-treated Tg(hsp:AML1-ETO) embryos. Scale bar, 0.3 mm. (d) In situ hybridization of mpo. Knockdown of ptgs2a or ptgs2b suppresses AE-induced mpo expression. Control, non-injected embryos. Scale bar, 0.3 mm.