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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Nat Chem Biol. 2009 Jan 26;5(4):236–243. doi: 10.1038/nchembio.147

Figure 6.

Figure 6

AE activates β-catenin-dependent transcription through COX-2. (a) Expression of AE induces TOPflash luciferase activity, which can be suppressed by overexpression of a dominant-negative form of TCF (dnTCF) or by NS-398 (75 μM). The empty vector (pCS2) or the expression vector for AE (pCS2-AE) was transfected into K562 cells along with TOPflash and pRL-tk which encodes Renilla luciferase for normalizing the transfection efficiency. The results are shown in relative TOPflash luciferase activities after normalization. pCS2/control, 1±0.09; pCS2+dnTCF, 0.73±0.03; pCS2+NS-398, 0.58±0.01; pCS2-AE/control, 12.29±1.09, pCS2-AE+dnTCF,2.93±0.3; pCS2-AE+NS-398, 5.29±0.57 (mean±SEM). *p<0.01 (two-tailed t-test). (b) 16,16-dimethylprostaglandin E2 (dmPGE2) activates β-catenin-TCF-dependent transcription. K562 cells were transfected with TOPflash and pRL-tk. At 4 hours after transfection, PGE2 (20 μM), dmPGE2 (20 μg ml−1) or DMSO was added to the cells. The luciferase activities were measured two days after the transfection. In addition, the dmPGE2-induced TOPflash activity can be suppressed by overexpression of dnTCF. control, 1±0.05; control+dn-TCF, 0.56±0.03; PGE2, 2.44±0.15; PGE2+dnTCF, 0.83±0.1; dmPGE2, 9.06±1.02; dmPGE2+dnTCF, 1.89±0.38 (mean±SEM). *p<0.01 (two-tailed t-test).