Figure 7.
Identification of the Glu-C fragment 65–87 of Hsp27 with pS82 by LC–MS analysis. The base ion chromatogram of the Lys-C plus Glu-C digest (A), the FTICR measurement of the precursor ion at 43.43 min (B), the CID-MS2 product ions of the precursor ion (m/z 795.7603) at 43.43 min (C), and the CID-MS3 product ions of the neutral loss ion (m/z 966.7) generated from the previous CID-MS2 fragmentation (D).