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. Author manuscript; available in PMC: 2009 Mar 20.
Published in final edited form as: J Proteome Res. 2008 Aug 23;7(10):4384–4395. doi: 10.1021/pr800376w

Table 1.

Estimated Stoichiometries (% ES) of pS78 and pS82 from IP of Hsp27 Before and After Thiolutin Treatmenta

SPAVAAPAYSRALS(78)RQLS(82)SGVSE
Groups No P Mono P Di-P (pS78+pS82)
IP using anti-Hsp27 Ab
Control 100% N.D. N.D.
Thiolutin-treated 32% 47% at pS82 16%
5% at pS78
IP using anti-pS78 Hsp27 Ab
Control N.D. N.D. N.D.
Thiolutin-treated 17% 10% at pS82 61%
12% at pS78
a

The %ES was calculated as the measured phosphopeptide divided by the sum of the same peptide with various forms. N.D. means not detected.