Table III.
Distribution of siRNA functionality for each target structure classification
| Guide Strand | |||||
|---|---|---|---|---|---|
| 5' | Central | 3' | Stem | ||
 |
5' | 35.4 | 43.2 | 40.9 | 18.9 |
| 0.4996 | 0.0141 | 0.0876 | |||
| 43 | 24 | 64 | 4 | ||
| Central | 41.6 | 49.9 | 50.7 | 56.0 | |
| 0.0149 | 0.4147 | 0.1948 | |||
| 31 | 41 | 29 | 1 | ||
| 3' | 35.9 | 49.5 | 36.7 | 70.2 | |
| 0.3969 | 0.0258 | 0.1405 | |||
| 64 | 19 | 50 | 4 | ||
| Stem | 45.0 | 52.1 | 49.9 | N/A | |
| 0.0035 | 0.4659 | 0.1011 | |||
| 66 | 23 | 71 | 0 | ||
Target structures were defined when 4 or more consecutive unpaired nucleotides were present in the specified regions of either the siRNA guide strand or the mRNA target. The entries in each box represent the average gene expression level (top), the p-value from a one-sided Student’s t-test (middle), and the total number of siRNAs within that group (bottom) for siRNAs in the Complete Dataset.