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Nuclear claudin-1 cannot increase the invasive ability of G361 melanoma cells.Stable transfectants of the empty vector (A), the PCDNA3.1-CLDN1 vector (B), and the PCDNA3.1-CLDN1 vector containing the S69A mutation (C) were created. These cells were then subjected to invasion through Matrigel in a modified Boyden chamber assay. Only the PCDNA3.1-CLDN1 stable transfectants demonstrated an increase in invasion (D).
