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Characterization of mouse RNR subunits. Different concentrations of R1 with 74 μg/ml R2 (A) or different concentrations of R2 with 110 μg/ml R1 (B) were assayed for 30 min incubation time in a total volume of 50 μl. As electron donor a Trx system containing 3 μm Trx1, 1 mm NADPH, and 0.1 μm TrxR was used.
