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D18G TTR and amyloidogenic M-TTRs are co-secreted with ΔKDEL BiP (BiPs). A, BiP constructs used in this study. B, BiP-myc, BiPs-myc, or control vector was transiently co-transfected with TTRs or M-TTRs in HeLa cells. After 24 h, medium was changed, and cells were maintained for another 24 h. Media were immunoprecipitated (IP) with anti-c-Myc antibody and analyzed by Western blotting (IB) with anti-TTR and anti-c-Myc antibodies. WT, wild type.
