FIGURE 3.

Relative protein expression level of pJHD500 in the K-12 BW25113 deletion backgrounds. A, proteins in crude cell extracts were separated by SDS-PAGE prior to exposure to a dilute polyclonal rabbit anti-luciferase serum. The primary antibody preparation reacts preferentially with the β-subunit and does not reflect true differences in the relative expression of α- and β-subunits. Lane 1, pJHD500 expressed in K-12 BW25113; lane 2, pJHD500 expressed in K-12 BW25113 ΔNfsA; lane 3: pJHD500 expressed in K-12 BW25113 Δfre. B, relative quantity of each protein was determined using LiCor densitometry software.