FIGURE 5.

SDS-PAGE analysis of oxidoreductase co-purification with luciferase. Approximately 1 mg of each oxidoreductase was incubated in a 10-ml solution containing immobilized luciferase. Oxidoreductase proteins were incubated with luciferase and repeatedly washed. Aliquots were visualized directly by SDS page and separately assayed using both the flavin injection and coupled bioluminescence assay (33, 34). All activity measured by the coupled assay was lost following the first wash step concurrent with the results obtained by SDS-PAGE. Approximate molecular weights are shown on the right portion of the panel.