Skip to main content
. 2009 Mar 27;284(13):8329–8336. doi: 10.1074/jbc.M807387200

FIGURE 1.

FIGURE 1.

D4 receptors reduce GABAAR currents and surface expression in PFC pyramidal neurons. A, plot of normalized peak GABA (50 μm)-evoked current as a function of time in the absence and presence of D4 agonist (PD168077, 30 μm) application in dissociated PFC pyramidal neurons. Inset, representative current traces (at time points denoted by #). Scale bar, 500 pA, 1 s. B, plot of normalized evoked IPSC as a function of time and agonist application in PFC slices. Inset, representative IPSC traces (at time points denoted by #). Scale bar, 25 pA, 20 ms. C, cumulative plot of the distribution of mIPSC amplitudes before (control) and after PD168077 application in a cultured PFC pyramidal neuron. Inset, representative mIPSC traces. Scale bar, 50 pA, 2 s. D, immunocytochemical images of surface GABAAR β2/3 subunits and MAP2 staining in PFC cultures either untreated (control) or treated with PD168077 (30 μm, 10 min). E, quantitative analysis of surface GABAAR β2/3 clusters along the dendrites (density, size, and normalized intensity) in control versus PD168077-treated neurons. *, p < 0.05, ANOVA. ctl or con, control; PD,. PD168077.