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Absorption and CD spectral changes upon addition of l-alanine to F152I-Mi. A, absorption spectra of 6 μm F152I-Mi (- - -) and of the enzyme plus 500 mm l-alanine (—) immediately (line 1) and after 0.6, 3, 8, 14, 26, 34, 60, 100, and 180 min (line 9). B, CD spectra of 5 μm F152I-Mi (- - -) and the enzyme plus 500 mm l-alanine (—) immediately (line 1) and after 8, 15, 23, 48, 68, 100, 125, and 206 min (line 9). In each case, the buffer used was 100 mm potassium phosphate, pH 7.4.
