TABLE 1.

Steady-state kinetic parameters of AGT-Ma, AGT-Mi, and variants (Fig. 2) for the pair alanine-glyoxylate

Enzyme	Substrate	Cosubstrate	kcat	Kml-alanine	Km glyoxylate	kcat/Km
			s-1	mm			s-1 mm-1
AGT-Ma	l-Alanine	Glyoxylate	45 ± 2a	31 ± 4a		1.4 ± 0.2a
	Glyoxylate	l-Alanine	45 ± 3a		0.23 ± 0.05a	196 ± 44a
AGT-Mi	l-Alanine	Glyoxylate	33 ± 5	28 ± 2		1.2 ± 0.2
	Glyoxylate	l-Alanine	37 ± 1		0.22 ± 0.01	168 ± 8
F152I-Mi	l-Alanine	Glyoxylate	33.6 ± 0.3	41 ± 1		0.82 ± 0.02
	Glyoxylate	l-Alanine	40 ± 2		0.25 ± 0.03	160 ± 21
F52I-Ma	l-Alanine	Glyoxylate	35 ± 1	37 ± 1		0.95 ± 0.04
	Glyoxylate	l-Alanine	39 ± 1		0.28 ± 0.04	139 ± 20
F152A-Ma	l-Alanine	Glyoxylate	21.2 ± 0.6	46 ± 3		0.46 ± 0.03
	Glyoxylate	l-Alanine	22.6 ± 0.3		0.34 ± 0.02	66 ± 4

^aFrom Ref. 4.