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Time course of MgATP-dependent [3H]MTX uptake by vacuolar membrane-enriched vesicles purified from liquid root cultures of atmrp1-1 (•) and wild-type Arabidopsis WS plants (○). MgATP-dependent MTX uptake from media containing 50 μm [3H]MTX was estimated as described in Fig. 3. Values shown are means ± S.E. (n = 3).
