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. 2009 Mar 27;284(13):8449–8460. doi: 10.1074/jbc.M808632200

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Copyright © 2009, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Net MgATP-dependent uptake of folate monoglutamate and folate polyglutamates by vacuolar membrane vesicles purified from DTY168/pYES3-AtMRP1 cells (A) and purified red beet vacuolar membrane vesicles (B). Net MgATP-dependent [³H]PteGlu₁ (100 μm)(□) or [³H]PteGlu_n (50 μm) uptake (), or [³H]PteGlu₁ (100 μm) uptake from assay media containing unlabeled PteGlu_n (50 μm)(▪) were measured after 10 and 20 min. In one set of experiments on red beet vacuolar membrane vesicles, the vanadate inhibitabilities of [³H]PteGlu₁ and [³H]PteGlu_n uptake and of [³H]PteGlu₁ uptake in the presence of unlabeled PteGlu_n were determined after the addition of 100 μm vanadate to the assay medium. Values shown are means ± S.E. (n = 3).

Inline graphic — Net MgATP-dependent uptake of folate monoglutamate and folate polyglutamates by vacuolar membrane vesicles purified from DTY168/pYES3-AtMRP1 cells (A) and purified red beet vacuolar membrane vesicles (B). Net MgATP-dependent [³H]PteGlu₁ (100 μm)(□) or [³H]PteGlu_n (50 μm) uptake (), or [³H]PteGlu₁ (100 μm) uptake from assay media containing unlabeled PteGlu_n (50 μm)(▪) were measured after 10 and 20 min. In one set of experiments on red beet vacuolar membrane vesicles, the vanadate inhibitabilities of [³H]PteGlu₁ and [³H]PteGlu_n uptake and of [³H]PteGlu₁ uptake in the presence of unlabeled PteGlu_n were determined after the addition of 100 μm vanadate to the assay medium. Values shown are means ± S.E. (n = 3).