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. 2009 Mar 27;284(13):8557–8566. doi: 10.1074/jbc.M806702200

FIGURE 2.

FIGURE 2.

Mapping the interaction domains on TRF1 and RLIM. A, schematic representation of the region of RLIM involved in binding to TRF1. B, the region of RLIM involved in binding to TRF1 was analyzed in a GST pulldown assay. H1299 cells were transfected with various RLIM-V5 mutants and subjected to immunoblotting analysis with anti-V5 antibody directly (left) or were first precipitated by GST or GST-TRF1 (right). C, H1299 cells transfected with FLAG-TRF1 were precipitated by GST or the various truncated GST-RLIM fusion proteins followed by immunoblotting with anti-FLAG antibody. D, schematic representation of TRF1 truncations fused to GST. E, GST or the various truncated GST-TRF1 fusion proteins were affinity-purified and incubated with H1299 cell extracts followed by detection of endogenous RLIM by immunoblotting with anti-RLIM.