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. 2009 Mar 27;284(13):8567–8581. doi: 10.1074/jbc.M808719200

FIGURE 5.

FIGURE 5.

Staining of ATF-2 phosphorylated at Ser-121 and at Ser-340. A, HeLa cells were double-stained with antibodies specific for ATF-2pS121 and with PI (for DNA). Peptide blocking was performed with the Ser(P)-121 peptide, the Ser-121 (nonphosphorylated) peptide, and the Ser(P)-340 peptide, as described in the text. Asterisks indicate cross-reactive staining in the perinuclear region. B, HeLa cells were double-stained with antibodies specific for ATF-2p340 and PI. Peptide blocking was performed with the Ser(P)-340 peptide, the Ser-340 (nonphosphorylated) peptide, and the Ser(P)-121 peptide, as described in the text. Scale bars, 10 μm.