Dose-response curves. The effective opening rate is the inverse of
the predominant (and slowest) intra-cluster nonconducting interval component,
whose high concentration asymptote is an estimate of the channel opening rate
constant. Top, εTrp-55 mutations. W (WT), dashed
black line (calculated from the rate constants in Tables
1 and
2); F, black; E,
red; A, blue; I, pink; V, gold; Y dark
red. Inset, C (cyan), S (purple), H
(green), and L (orange) mutants showed insufficient
saturation to estimate an asymptote. Bottom, δTrp-57 mutations.
F, black; E, red; A, blue; V, gold;Y,
dark red; H, green; L, orange; R, dark
pink.