TABLE 1.
Construct
|
Rate constant
|
Equilibrium constant
|
||||
---|---|---|---|---|---|---|
f2 | b2 | E2 | Fold change | ΔΔG | ||
s–1 | kcal mol–1 | |||||
WTa | 48,000 | 1700 | 28.2 | |||
εW55A | 3860 | 1970 | 1.9 | 14.4 | +1.6 | |
εW55E | 8670 | 1960 | 4.4 | 6.4 | +1.1 | |
εW55F | 5440 | 1200 | 4.5 | 6.2 | +1.1 | |
εW55I | 32,660 | 1730 | 18.9 | 1.5 | +0.2 | |
εW55V | 5190 | 3120 | 1.7 | 17.0 | +1.7 | |
εW55Y | 4360 | 1670 | 2.6 | 10.8 | +1.4 | |
δW57A | 29,510 | 1720 | 17.2 | 1.6 | +0.3 | |
δW57E | 19,050 | 810 | 23.6 | 1.2 | +0.1 | |
δW57F | 11,880 | 1690 | 7.0 | 4.0 | +0.8 | |
δW57H | 14,720 | 1830 | 8.0 | 3.5 | +0.7 | |
δW57L | 14,000 | 1990 | 7.0 | 4.0 | +0.8 | |
δW57R | 5490 | 3280 | 1.7 | 16.9 | +1.7 | |
δW57V | 5670 | 1980 | 2.9 | 9.8 | +1.4 | |
δW57Y | 26,910 | 1650 | 16.3 | 1.7 | +0.3 |
Data are from Ref. 39