TABLE 2.
Construct
|
Rate constant
|
Equilibrium constant
|
|||
---|---|---|---|---|---|
k+ | k– | Kd | Fold change | ΔΔG | |
μm–1 s–1 | s–1 | μm | kcal mol–1 | ||
WTa | 170 | 24,750 | 150a | ||
εW55A | 5 | 17,890 | 3740 | 25.3 | +1.9 |
εW55E | 11 | 17,950 | 1610 | 10.8 | +1.4 |
εW55F | 27 | 16,500 | 620 | 4.2 | +0.8 |
εW55I | 20 | 66,310 | 3320 | 22.4 | +1.8 |
εW55V | 27 | 30,640 | 1560 | 10.5 | +1.4 |
εW55Y | 37 | 10,460 | 280 | 1.9 | +0.4 |
δW57A | 6 | 26,770 | 4170 | 28.1 | +1.9 |
δW57Fb | 135 | 30,590 | 230 | 1.5 | +0.2 |
δW57H | 57 | 17,600 | 310 | 2.1 | +0.4 |
δW57L | 40 | 70,980 | 1730 | 11.7 | +1.4 |
δW57R | 21 | 15,570 | 750 | 5.0 | +0.9 |
δW57V | 27 | 17,160 | 470 | 3.2 | +0.7 |
δW57Y | 20 | 30,110 | 1510 | 10.2 | +1.3 |