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TP did not block binding of pemphigus IgG to Dsg1 and Dsg3. ELISA experiments with recombinant Dsg3 (black bars) and Dsg1 (white bars) revealed that autoantibody scores of PV-IgG 1, PV-IgG 2, and AK 23 in the absence or presence of TP (20 μm) were not significantly different (p = 0.4040, p = 0.751, p = 0.2624, respectively), indicating that TP did not interfere with binding of pemphigus IgG to Dsg1 and Dsg3, respectively. (n = 3 for each condition).
