FIGURE 7.

Ilicicolin binding rates at different inhibitor or DBH₂ concentrations. In A, the rates of ilicicolin binding obtained in the absence of center P inhibitor (squares) with myxothiazol (triangles), as well as the initial rate in the presence of stigmatellin (solid circles), were fitted to a linear regression (solid lines) to obtain the binding rate constant for ilicicolin (k_on). The slower ilicicolin binding rates obtained with stigmatellin at center P(open circles) showed a very weak concentration dependence, as shown by the very low apparent k_on value obtained by a linear fit (dashed line). B shows the binding rate of 60 μm ilicicolin to center N in the presence of different DBH₂ concentrations and in the absence of center P inhibitors.