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Induction of DNA single strand breaks and oxidized bases by menadione measured using the comet assay (single strand breaks) (A) and Fpg FLARE comet assay (oxidized bases) (B). Cells were treated with various concentrations of menadione for 90 min in S30 and MDA-MB-231 cells. Closed diamonds, menadione in S30 cells; open diamonds, menadione + 4-OHTAM (10 nm, 1 h) in S30 cells; open circles, menadione in MDA-MB-231 cells. Data represent the mean ± S.E. (n = 3).
