TABLE 1.

LC-MS/MS analysis of 8-oxo-dG in S30 and MDA-MB-231 cells treated with 4-OHEN or menadione Cells were treated with 4-OHEN or menadione (10 μm) for 90 min with or without 4-OHTAM (10 nm) pretreatment for 60 min. Experimental details are described under “Experimental Procedures.” Data represent the mean ± S.D. (n = 2). ^*, significant difference (p < 0.05) compared with the S30 sample treated with 4-OHEN only.

Treatment	8-Oxo-dG/dG × 105
		4-OHEN		Menadione
	S30	MDA-MB-231	S30	MDA-MB-231
DMSO	1.06 ± 0.04	0.98 ± 0.04	1.20 ± 0.25	1.10 ± 0.21
10 μm	1.65 ± 0.16	1.37 ± 0.08*	2.26 ± 0.41	2.23 ± 0.07
10 μm + 4-OHTAM	1.29 ± 0.01*	1.34 ± 0.13	2.40 ± 0.51	2.62 ± 0.14