TABLE 2.
A cHx1-cP51A double mutant is highly deficient in growth on malate and β-subunit content, and a single cG17A mutant alone exhibits significant defects
|
Growtha,b
|
β-Subunit content
(Western)a,c
|
||||
|---|---|---|---|---|---|
| Strain |
Glucose
|
Malate
|
|||
| pH 7.5 | pH 10.5 | pH 7.5 | pH 10.5 | ||
| % | |||||
| Wild-type | 100 | 100 | 100 | 100 | 100 |
| cHx1 | 105 ± 18 | 107 ± 10 | 0 | 0 | 4 ± 0.1 |
| cP51A | 62 ± 9 | 89 ± 13 | 75 ± 6 | 23 ± 10 | 73 ± 4 |
| cHx1-cP51A | 110 ± 3 | 101 ± 3 | 0 | 0 | 6 ± 0.4 |
| cG17A | 103 ± 7 | 76 ± 13 | 55 ± 18 | 60 ± 11 | 65 ± 12 |
a
% of wild-type.
b
Average of two independent experiments ± S.D.
c
Average of two vesicle preparations ± S.D.