FIGURE 6.

Talin IBS2 but not IBS2-A or IBS2-B binds to acidic phospholipids. Binding of His-tagged talin polypeptides to phosphatidylinositol phosphate strips containing an array of acidic phospholipids (Invitrogen) was detected using an anti-His antibody. Each spot contains 100 pmol of phospholipid, and the membrane was challenged with 1 μg/ml protein. Talin IBS2 (residues 1974-2293) binds to several phospholipids, whereas the individual five-helix bundles that make up IBS2, i.e. residues 1974-2140 (IBS2-A) and residues 2137-2293 (IBS2-B), did not bind to any of the phospholipids tested.