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. 2009 Mar 27;284(13):8898–8909. doi: 10.1074/jbc.M804281200

FIGURE 2.

FIGURE 2.

Ultrasound stimulates focal adhesion formation independently of syndecan-4 expression. Syndecan-4-/- MEFs were spread on 50K for 2 h before stimulating with syndecan-4 ligand (H/0) for 40 min either with (closed bars) or without (open bars) ultrasound for 20 min. A, representative cells stained for vinculin (green) and actin (red). Bar, 10 μm. B, 400 cells/condition were scored for focal adhesion formation. Total focal adhesion area (C) or total focal adhesion integrated density (D) of 25-35 syndecan-4-/- cells/condition was measured using image J software. Error bars, S.E.; significance values are as follows: *, p < 0.05; **, p < 0.01; ***, p < 0.001. Images and analyses are representative of experiments performed on at least three separate occasions.