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. 2009 Mar 27;284(13):8984–8994. doi: 10.1074/jbc.M809719200

FIGURE 1.

FIGURE 1.

Recombinant αLNNd and mAgrin. Panel A, αLNNd and mAgrin. The chimeric protein αLNNd is composed of the N-terminal LN and LEa domains of the laminin α1 subunit (containing laminin polymerization (P) activity) fused to the C-terminal G2, LE, and G3 domains of nidogen-1 (containing type IV collagen-binding (C4) and laminin γ1-binding (Lm) activities). The internally truncated protein mAgrin consists of the laminin coiled-coil binding NtA domain fused through the first follistatin (FS) domain to the dystroglycan and sulfatide binding (DG/S) terminal laminin-like LG and LE domain complex. Panel B, Coomassie Blue-stained gels (SDS-PAGE, 8% acrylamide, reducing conditions) of mAgrin, αLNNd, LmΔ(αLN&LG), LmΔαLN, and (wt) laminin-111. Panel C, diagrammatic representations of the recombinant heterotrimeric laminins used in this study.